Karakteristik Bakteri Pendegradasi Bahan Pangan Asal Saluran Pencernaan Kecoa Amerika (Periplaneta americana) dari Pasar Tradisional
DOI:
https://doi.org/10.29080/biotropic.v7i1.1662Keywords:
Bakteri amilolitik, bakteri proteolitik, bakteri lipolitik, Kecoa Amerika, bahan panganAbstract
American cockroach (Periplaneta americana) is one of the most common pests found in houses, apartments, and public facilities such as markets. As vector insects, cockroaches have symbiosis with microorganisms. Some of these microorganisms can live in cockroaches' cuticles and digestive tracts. Besides being able to pose a danger to human health, the microbes in cockroach bodies also have the potential to cause food damage. This study aimed to determine the diversity of microorganisms originating from the digestive tract of the American cockroach and their potential to cause food spoilage. The research was conducted in several stages: sampling cockroaches at a traditional market, isolating bacteria from the digestive tract of cockroaches, qualitatively testing amylolytic, proteolytic, and lipolytic potentials, and characterizing bacterial isolate by morphologically and biochemically character. The results showed that there were 26 isolates of bacteria isolated from the digestive tract of American cockroaches from the market, 62.96% of the isolates were found to have the ability to degrade protein, 37.04% of the isolates had the ability to degrade starch, and 40.74% of the total isolates can degrade fat. These isolates had various morphological and biochemical characteristics. Bacterial isolates derived from American cockroaches from traditional markets have the potential to degrade food.
Downloads
References
Amalia, H., & Harahap, I. S. 2010. Preferensi Kecoa Amerika (Periplaneta
americana) (Blattaria : Blattidae) terhadap Berbagai Kombinasi Umpan.
Entomol, 7(2), 67–77.
Arini, L.D.D., 2017. Faktor-Faktor Penyebab dan Karakteristik Makanan Kadaluarsa yang Berdampak Buruk pada Kesehatan Masyarakat. Jurnal Teknologi dan Industri Pangan, 2 (1),15-24
Bell WJ, Roth LM, Nalepa CA, & Wilson EO. 2007. Cockroaches: ecology, behavior, and natural history. Johns Hopkins University Press, p 248
Cahyani, Kartika L., Yuliawati, S., & Martini. 2018. Gambaran Faktor-Faktor Yang Terkait Dengan Kepadatan Kecoa di Tempat Penjualan Bahan Pangan dan Makanan Pasar Tradisional Kota Semarang. Jurnal Kesehatan Masyarakat (e-Journal) 6(5), 295-301
Cochran, D.G. 1999. Cockroaches : Their Biology, Distribution and Control. World Health Organization
Guzman, J., & Vilcinskas, A. 2020. Bacteria associated with cockroaches: health risk or biotechnological opportunity?. Applied Microbiology and Biotechnology, 04, 10369–10387
Khatoon, H., Anokhe, A., & Kalia, Vinay. 2022. Catalase Test: A Biochemical Protocol for Bacterial Identification. AgriCos e-Newsletter 3 (1), 53-55
Kundera, I.N., Sapu, E.H., & Bialangi, M.S. 2020. Identification of Bacteria on Cockroach Feet (Periplaneta americana) in Resident Bay of Palu Permai and Sensitivity Test Against Antibiotics. Techno: Jurnal Penelitian, 9(1), 535-362
Menasria, T., Moussa, F., El-Hamza, S., Tine, S., Megri, R., & Chenchouni, H. 2014. Bacterial load of German cockroach (Blattella germanica) found in hospital environment. Pathogens and global health, 108(3), 141–147.
Moges F., Eshetie, S., Endris, M., Huruy, K., Muluye, D., Feleke, T., G/Silassie, F., Ayalew, G., & Nagappan, R. 2016. Cockroaches as a Source of High Bacterial Pathogens with Multidrug Resistant Strains in Gondar Town, Ethiopia. BioMed Research International, 2016 : 1-6
Mpuchane, S., Allotey, J., Matsheka, M., Simpanya, M., Coetzee, S., Jordaan, A., Mrema, N. & Gashe, B.A.. 2006. Carriage of micro-organisms by domestic cockroaches and Implications on food safety. International Journal of Tropical Insect Science. 26(3), 166 - 175.
Murray, R. K., D. K. Granner, P. A. Mayes & V. W. Rodwell. 1999. Biokimia Harper. Penerbit buku kedokteran EGC. Jakarta.
Nalepa C, Bignell D, Bandi C. 2001. Detritivory, coprophagy, and the evolution of digestive mutualisms in Dictyoptera. Insect Soc. 48(3), 194–201.
Ni’matuzahroh, Trikurniadewi, N., Ibrahim, S. N. M. M., Abidin, A. Z., Khiftiyah, A. M., Sari, S. K., Nuswantara, E. N., Nurmansyah, F., Rahman, M. A. R. W., Maghfirah, H. L., Jannah, M., Masrurin, A. R., Saidah, L., Makrifah, R. L., Fatimah, & Affandi, M. 2020. Isolation and characterization of cockroach endosymbiont bacteria with potential to produce hydrolytic enzyme of organic material. Ecology, Environment and Conservation. 26, 123-131.
Patel, M., Mistry, J., Desai, S., Patel, S., & Desai, S. 2016. Isolation and Characterization of Lipase producing Bacteria from Vegetable Oil Spillage Site. International Journal of Current Microbiology and Applied Sciences. 5. 214-232.
Putri, W.D.R., Marseno D.W., & Cahyanto, M.N. 2012. Isolasi dan Karakterisasi Bakteri Asam Laktat Amilolitik selama Fermentasi Growol, Makanan Tradisional Indonesia. Jurnal Teknologi Pertanian. 1(13), 52-60.
Ramsay, C.A. & Thomasson, G.L. 2009. Public Health Pest Control, Cooperation Extension, Washington State University, Washington
Ray, Bibek. 2005. Fundamental food microbiology 3rd Edition. CRC Press. Washington DC.
Rorong, J. A., & Wilar, W. F. 2021. Keracunan Makanan oleh Mikroba. Techno Science Journal, 2(2), 47-60.
Setyati, W. A., & Subagiyo, D. 2012. Isolasi dan Seleksi Bakteri Penghasil Enzim Ekstraseluler (proteolitik, amilolitik, lipolitik dan selulolitik) yang Berasal dari Sedimen Kawasan Mangrove. Jurnal Ilmu Kelautan, 17(3), 164–168.
Shields, P., & Cathcart, L. 2010. Oxidase Test Protokol. American Society for Microbiology. diakses pada laman: https://asm.org/getattachment/00ce8639-8e76-4acb-8591-0f7b22a347c6/oxidase-test-protocol-3229.pdf
Sucipto, D.C. 2011. Vektor Penyakit Tropis. Yogyakarta: Gosyen Publishing
Sukarminah, E., D.M. Sumanti & I. Hanidah. 2010. Mikrobiologi Pangan Jurusan Teknologi Industri Pangan. Fakultas Teknologi Industri Pertanian. Universitas Padjajaran. Jatinagor
Zainuddin, M., Setyati, W.A., Person, D., & Renta, P. 2017. Zona Hidrolisis dan Pertumbuhan Bakteri Proteolitik dari Sedimen Ekosistem Mangrove Rhizophora mucronata Telukawur Jepara. Jurnal Sumberdaya Perairan, 11(2), 31-35.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 dwiana muflihah yulianti, Arif Rahman Hikam, Trisnowati Budi Ambarningrum, Taruna Dwi Satwika, Dyah Fitri Kusharyati, Hilmiyah Al'alawiyah

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.