Uji Toksisitas Air Limbah Deterjen Terhadap Larva Artemia salina Leach Dengan Metode Brine Shrimp Lethality Test (BSLT)

Abstract

Detergent waste water contains ABS (Alkyl Benzene Sulphonate), classified as a detergent which can cause environmental pollution and difficult to decompose ecosystems in the waters. This study aims to test the toxicity of detergent waste against Artemia salina Leach larvae and determine the LC₅₀ value. The study was conducted using a completely randomized design of 5 groups with 10 Artemia salina Leach larvae in each group with three repetitions. The concentrations used were 0 mg/mL, 10 mg/mL, 50 mg/mL, 500 mg/mL and 1000 mg/mL. Mortality observation of Artemia salina Leach larvae was carried out with 3 replications at 6 hours, 12 hours, 24 hours and 48 hours. At a concentration of 0 mg/mL there was no death in the larvae either at 6 hours, 12 hours, 24 hours and 48 hours. At concentrations of 10 mg/mL, 50 mg/mL, 500 mg/mL and 1000 mg/mL resulted in the lowest mortality rate for larvae at 6 hours with successive results of 0%, 10%, 30% and 40% while the The highest mortality was in larvae at 48 hours with successive results of 50%, 50%, 66%, 67% and 100%. The results showed that the probit analysis obtained an LC₅₀ value at 24 hours of 0.004132 mg/mL, while at 48 hours it was 0.00135706 mg/mL.