Biotropic : The Journal of Tropical Biology 2022-09-01T09:45:43+00:00 Hanik Faizah Open Journal Systems <p><strong>Biotropic : The Journal of Tropical Biology</strong>&nbsp;publish articles on South East Asia Tropical Biology from various perspectives, covering both literary and fieldwork studies. The Journal put empahis on aspects related to:<br>Ecology<br>Zoology<br>Botany<br>Microbiology<br>Biology molecular<br>Biospeleology<br>Conservation and other&nbsp; tropical biology discourses</p> <p>Journals are published biannually in February and August. Editor invite all relevant parties to contribute in publishing sciencetific articles which have never been published&nbsp;</p> Optimasi Suhu dan Waktu SSF untuk Produksi Bioetanol dari Sampah Daun Menggunakan Trichoderma Viride dan Zymomonas Mobilis 2022-07-24T01:13:54+00:00 Wulan Fitriani Safari <p><em>Foliage from gardens or parks is biomass whose organic fraction can be decomposed. Foliage can be utilized by converting it into bioethanol. Various studies have shown that Trichoderma viride and Zymomonas mobilis play a role in the production of bioethanol from biomass, but until now there has been no report on the production of bioethanol from foliage using a mixture of T. viride and Z. mobilis. The purpose of this study was to determine the optimal SSF temperature and time for bioethanol production from foliage using T. viride and Z. mobilis. The fermentation process used the Simultaneous Saccharification and Fermentation (SSF) method with a mixture of T. viride and Z. mobilis with a composition of 5% : 5% (w/v). SSF was carried out at several variations of time and temperature, 60 hours, 72 hours, 80 hours and 96 hours for times and temperatures used are 32<sup>o</sup>C, 35<sup>o</sup>C and 38<sup>o</sup>C. The fermented bioethanol was purified by distillation and finally, the ethanol content was tested with GC. Bioethanol was successfully produced from foliage using T. viride and Z. mobilis with the highest ethanol content obtained in SSF with a temperature of 35<sup>o</sup>C and a time of 72 hours, which is 0.2151%.</em></p> 2022-08-31T00:00:00+00:00 Copyright (c) 2022 Wulan Fitriani Safari Chromosome Characterization of Brassicaceae Family 2022-08-09T00:36:18+00:00 Ganies Riza Aristya Bening Larasati Galang Riswi Dyatama Himawan Masyhuri Febri Yuda Kurniawan Fauzana Putri Dian Astriana Yuanita Rachmawati <p><em>Indonesia is known as a rich country in various agricultural and plantation products, including</em> <em>vegetables such as mustard, broccoli, cabbage, and cauliflower. However, in its cultivation,</em> <em>the products and demand for vegetables are not offset by an increase in the production quality. One of the efforts to improve and enhance the production quality is to identify and characterize chromosomes of plants which will become the basis for plant breeding activities. The purpose of this study was to characterize the number, form and size of the chromosome in cultivars belonging to</em> <em>the Brassicaceae family. The study was carried out using the modified squash method. Chromosomes were prepared by fixation, maceration, and staining, then the mitotic phases were observed using a microscope and optilab, and analyzed using Image Raster 3. The results showed that mitotic time range and chromosome characte</em><em>r</em><em> of six cultivars of the Brassicaceae family were different. Broccoli</em><em> (</em><em>'Chief No.</em> <em>2 1955' and 'Green Super') and cauliflower </em><em>(</em><em>'ILONA' and 'TM 126') had a mitotic time range from 04.00 to 09.00 a.m. with 2n chromosome number = 18. </em><em>Green mustard </em><em>(</em><em>‘Juwita’</em><em> and </em><em>‘TM Jade’) and white mustard </em><em>(</em><em>'Sakata' and 'Shuka-shuka')</em><em> had a mitotic time range from 03.00 to 08.00 a.m. with 2n chromosome number = 20. White cabbage </em><em>(</em><em>‘CR ACE' and 'Sehati F<sub>1</sub>') had a mitotic time range from 04.00 to 09.00 a.m and red cabbage </em><em>(</em><em>‘Scarlet’ and ‘Red Globe’) had a mitotic time range from 09.00 to 10.00 a.m. with 2n chromosome number</em> <em>=</em> <em>18.</em></p> <p><strong><em> </em></strong></p> <p> </p> 2022-08-31T00:00:00+00:00 Copyright (c) 2022 Ganies Riza Aristya, Yuanita Rachmawati Uji Antagonis Bakteri Simbion Spons dari Pulau Bakar terhadap Jamur Candida Albicans 2022-07-24T01:15:38+00:00 Rizki Amelia Nasution Ulfayani Mayasari <p><em>Sponge symbiont bacteria are microbes that live in marine sponges and are known to have bioactive compounds with </em>antimicrobial <em>propeties</em><em>.</em><em> Sea sponges on Bakar Island are known to contain many types of symbiotic bacteria. This study was conducted to test the ability of the sponge symbiont bacteria to inhibit the growth of the fungus Candida albicans. The study was carried out in 3 stages, with were sample collection, isolation and antifungal activity test using the </em><em>well diffusion technique</em><em>. Sponge symbiont bacteria were obtained as many as 16 isolates and were successfully tested for as many as 11 isolates for their antifungal abilities. Of the 11 isolates, based on the characteristics tested, 1 isolate (SPS4F2) was obtained with strong criteria (12.06 mm) and 10 </em><em>(</em><em>SPS1, SPS3, SPS5, SPS6, SPS7 SPS8, SPS9, SPS10, SPS11, SPS12, SPS13) </em><em>others with moderate criteria (±6.00-9.00).</em> <em>The results of the study can be concluded that the sponge symbiont bacteria from Bakar’s Island mostly have the potential as antifungals against Candida albicans and based of characterization including the Bacillus.</em></p> 2022-08-31T00:00:00+00:00 Copyright (c) 2022 Rizki Amelia Nasution, Ulfayani Mayasari Identifikasi Bakteri Toleran terhadap Logam Berat Pb yang Diisolasi dari Air dan Sedimen di Sungai Porong, Sidoarjo 2022-08-27T05:27:08+00:00 Nur Rokhmatul Lailiya Misbakhul Munir Esti Tyastirin Eko Teguh Pribadi Hanik Faizah <p><em>Porong River is an active river located in the Sidoarjo region, East Java which has been polluted by the Lapindo mudflow containing various chemical compounds, one of which is lead (Pb) heavy metal. Isolation of bacteria in the environment contaminated with Pb allows bacteria that have the potential as bioremediation agents to grow. The purpose of this study was to determine the content of heavy metal Pb and isolate and identify lead (Pb) tolerant bacteria in water and sediment at Porong river, Sidoarjo. Sampling of water and sediment was carried out at 2 sampling points based on the presence of Lapindo mud waste flow. Analysis of the heavy metal content of Pb was carried out using the Atomic Absorption Spectrophotometer (AAS). Bacterial isolation was carried out on NA media containing 10 ppm Pb of heavy metal and incubated for 24 hours at 30<sup>0</sup>C. Bacterial isolates were identified based on the macroscopic and microscopic properties and biochemical tests. The results of the heavy metal content of Pb at sampling point 1 in water was 0.81 ppm, and in sediment was 0.98 ppm while at sampling point 2 in water was 2.93 ppm, and in sediment was 3.88 ppm. The results of bacterial identification obtained as many as 6 bacterial isolates belonging to 3 genera of bacteria including Bacillus, Acinetobacter and Pseudomonas.</em></p> 2022-08-31T00:00:00+00:00 Copyright (c) 2022 Nur Rokhmatul Lailiya , Misbakhul Munir, Esti Tyastirin, Eko Teguh Pribadi, Hanik Faizah Analisis Kuantitatif Kandungan Antioksidan dan Aktivitas Sitotoksik dari Ekstrak Anastatica hierochuntica L 2022-08-30T10:43:10+00:00 Nova Lusiana Risa Purnamasari Eva Agustina Nurul Ilmi Faidah Azlinda Mitha Agustin Muhammad Falikhul M <p><em>Antioxidants are compounds that can counteract free radicals in the body due to the presence of active compounds such as flavonoids and phenolics. These active compounds can be cytotoxic, ie, compounds capable of inhibiting and stopping the growth of cancer cells by using toxicity test by BSLT (Brine Shrimp Lethality Test) method. This study aims to determine the antioxidant activity by DPPH (Dipheny Pycryl Hydrazil) method and toxicity test by BSLT method of Anastatica hierochuntica L extract. A. hierochuntica L extraction were done by maceration method with methanol solvent. The content of A. hierochuntica L extract compounds was done by phytochemical test to know flavonoid compounds, triterpenoid and sterol. Meanwhile, alkaloid and saponin and liquid chromatography to see flavonoid compounds of quersetin. Furthermore, the antioxidant activity test was carry out by the DPPH method and by BSLT method to observed the mortality of shrimp larvae. A. hierochuntica extract has antioxidant activity with IC50 value 77,23 μg / ml and toxicity value of LC50 44,97. A.hierochuntica L extracts potentially have antioxidant and cytotoxic properties.</em></p> 2022-08-31T00:00:00+00:00 Copyright (c) 2022 Nova Lusiana, Risa Purnamasari, Eva Agustina Efektivitas Penggunaan Media Tanam Berbasis Agen Bioteknologi Terhadap Pertumbuhan Tanaman Hias Episcia cupreata 2022-07-28T04:57:51+00:00 Isnin Meiva Ida Yayu Nurul Hizqiyah Cartono <p><em>Planting media is the most important aspect for plant growth. However, in fact, agricultural land in Indonesia is of relatively low quality. These factors include nutrient deficits, decreased levels of organic matter, pollution, decreased microbial activity, and salinization/alkalinization. This study aims to determine the effectiveness of the use of planting media based on biotechnology agents on the growth of Episcia cupreata. The method is a quasi-experimental, RAK design (Randomized Block Design) with 6 treatments, 4 treatments. Main data analysis with ANOVA test and DMRT test level 5%</em> <em>and supporting data with correlation analysis. The result shows the effectiveness of using planting media based on biotechnology agents on the growth of Episcia cupreata. The most optimal growth of the number of leaves produced by treatment T1 with an average of 19.75, stem height by treatment T2 with an average of 48 cm, and root length by treatment T2 with an average of 10</em><em>,27</em><em> cm</em><em>. Physicochemical conditions include soil pH, temperature, air humidity</em><em>,</em><em> soil moisture, and light intensity were correlated with the growth of Episcia cupreata.</em></p> 2022-08-31T00:00:00+00:00 Copyright (c) 2022 Isnin Meiva