Analisis Kuantitatif Kandungan Antioksidan dan Aktivitas Sitotoksik dari Ekstrak Anastatica hierochuntica L

Authors

  • Nova Lusiana Program Studi Psikologi, Fakultas Psikologi dan Kesehatan, UIN Sunan Ampel, Surabaya, Indonesia
  • Risa Purnamasari Program Studi Biologi, Fakultas Sains dan Tekhnologi, UIN Sunan Ampel, Surabaya, Indonesia
  • Eva Agustina Program Studi Biologi, Fakultas Sains dan Tekhnologi, UIN Sunan Ampel, Surabaya, Indonesia
  • Nurul Ilmi Faidah Program Studi Biologi, Fakultas Sains dan Tekhnologi, UIN Sunan Ampel, Surabaya, Indonesia
  • Azlinda Mitha Agustin Program Studi Biologi, Fakultas Sains dan Tekhnologi, UIN Sunan Ampel, Surabaya, Indonesia
  • Muhammad Falikhul M Program Studi Biologi, Fakultas Sains dan Tekhnologi, UIN Sunan Ampel, Surabaya, Indonesia

Keywords:

Antioxidant, Cytotoxic, Anastatica hierochuntica L

Abstract

Antioxidants are compounds that can counteract free radicals in the body due to the presence of active compounds such as flavonoids and phenolics. These active compounds can be cytotoxic, ie, compounds capable of inhibiting and stopping the growth of cancer cells by using toxicity test by BSLT (Brine Shrimp Lethality Test) method. This study aims to determine the antioxidant activity by DPPH (Dipheny Pycryl Hydrazil) method and toxicity test by BSLT method of Anastatica hierochuntica L extract. A. hierochuntica L extraction were done by maceration method with methanol solvent. The content of A. hierochuntica L extract compounds was done by phytochemical test to know flavonoid compounds, triterpenoid and sterol. Meanwhile, alkaloid and saponin and liquid chromatography to see flavonoid compounds of quersetin. Furthermore, the antioxidant activity test was carry out by the DPPH method and by BSLT method to observed the mortality of shrimp larvae. A. hierochuntica extract has antioxidant activity with IC50 value 77,23 μg / ml and toxicity value of LC50 44,97. A.hierochuntica L extracts potentially have antioxidant and cytotoxic properties.

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References

Astarina, N. W. G., Astuti, K. W., & Warditiani, N. K. (2013). SKRINING FITOKIMIA EKSTRAK METANOL RIMPANG BANGLE. https://ojs.unud.ac.id/index.php/jfu/article/view/7399

Duke, J. A. (2007). Duke’s Handbook of Medicinal Plants of the Bible. CRC Press.

Frengki, F., Roslizawaty, R., & Pertiwi, D. (2014). Toxicity Test of Ethanol Extract Ant Plant Local Aceh (Mymercodia sp) Method of BSLTLarvae Shrimp Artemia salina Leach. Jurnal Medika Veterinaria, 8(1).

Ganong, W. F. (1921). A Textbook of Botany for Colleges. MacMillan Company.

Hadi Sunaryo, H., & Priyanto, P. (2018). TOKSIKOLOGI Mekanisme, Terapi Antidotum, dan Penilaian Risiko. http://repository.uhamka.ac.id/517/

Hardiana, R., & Rudiyansyah, T. A. (2012). Aktivitas antioksidan senyawa golongan fenol dari beberapa jenis tumbuhan Famili Malvaceae. Jurnal Kimia Khatulistiwa, 1(1).

Ihsanullah, D. (2012). Chemical properties of the medicinal herb Kaff Maryam (Anastatica hierochuntica L.) and its relation to folk medicine use. African Journal of Microbiology Research, 6(23), 5048–5051.

Ivanišová, E., Tokár, M., Mocko, K., Bojňanská, T., Mareček, J., & Mendelová, A. (2020). ANTIOXIDANT ACTIVITY OF SELECTED PLANT PRODUCTS. Journal of Microbiology, Biotechnology and Food Sciences, 2020/21(vol. 10), 1692–1703.

Julfitriyani, J. (2016). UJI AKTIVITAS ANTIOKSIDAN DAN TOKSISITAS EKSTRAK ETANOL DAUN FOKI SABARATI (Solanum Torvum. PHARMACON, 5(3).

Kartikasari, D. (2010). Pengaruh Penggunaan Media Yang Berbeda Terhadap Kemampuan Penyerapan Logam Berat Pb Pada Nannochloropsis sp. Dalam Skripsi. Fakultas Pertanian Universitas Lampung.

Kikuzaki, H., Hisamoto, M., Hirose, K., Akiyama, K., & Taniguchi, H. (2002). Antioxidant properties of ferulic acid and its related compounds. Journal of Agricultural and Food Chemistry, 50(7), 2161–2168.

Md Zin, S. R., Mohamed, Z., Alshawsh, M. A., Wong, W. F., & Kassim, N. M. (2018). Mutagenicity evaluation of Anastatica hierochuntica L. aqueous extract in vitro and in vivo. Experimental Biology and Medicine, 243(4), 375–385.

Meyer, B., Ferrigni, N., Putnam, J., Jacobsen, L., Nichols, D. j, & McLaughlin, J. L. (1982). Brine shrimp: A convenient general bioassay for active plant constituents. Planta Medica, 45(05), 31–34.

Molyneux, P. (2004). The Use of the Stable Free Radical Diphenylpicryl-hydrazyl.

Mutia, D. (2010). Uji toksisitas Akut Ekstrak Etanol Buah Anggur (vitis vinifera) terhadap Larva Artemia salina Leach dengan Metode Brine shrimp Lethality test (Bst.

Nani, D. (2009). Pengaruh Air Rendaman Rumput Fatimah (Anastatica Hierochuntica L) terhadap Frekuensi Kontraksi Otot Uterus Tikus Galur Sprague Dawley pada Fase Estrus. Jurnal Keperawatan Soedirman, 4(1), 1–8.

Noviyanti, N., Herman, R. B., & Serudji, J. (2017). Pengaruh Pemberian Air Rendaman Rumput Fatimah (Anastatica Hierochuntica) Terhadap Kadar Hormon Estrogen Pada Tikus Putih (Rattus Norvegicus) Bunting. AcTion: Aceh Nutrition Journal, 2(2), 109–113.

Rita, W. S., Suirta, I. W., & Sabikin, A. (2008). Isolasi dan Identifikasi senyawa yang berpotensi sebagai antitumor pada daging buah pare (Momordica charantia L. Jurnal Kimia (Journal of Chemistry.

Sayuti, K., & Yenrina, R. (2015). Antioksidan alami dan sintetik. Padang. Universitas Adalas.

Siagian, A. (2002). Bahan Tambahan Makanan. Fakultas Kesehatan Masyarakat Universitas Sumatera Utara.

Suhirman, S., Hernani, H., & Syukur, C. (2006). UJI TOKSISITAS EKSTRAK LEMPUYANG GAJAH (Zingiber zerumbet) TERHADAP LARVA UDANG. Artemia salina Leach.

Umayah, E., & Amrun, M. (2007). Uji Aktivitas Antioksidan Ekstrak Buah Naga (Hylocereus Undatus (Haw. Britt. & Rose). Jurnal Ilmu Dasar, 8(1), 83–90.

Wickens, G. E. (1998). Ecophysiology of Economic Plants in Arid and Semi-Arid Lands. Springer-Verlag. https://doi.org/10.1007/978-3-662-03700-3

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Published

2022-08-31

How to Cite

Lusiana, N., Purnamasari, R., Agustina, E., Nurul Ilmi Faidah, Azlinda Mitha Agustin and Muhammad Falikhul M (2022) “Analisis Kuantitatif Kandungan Antioksidan dan Aktivitas Sitotoksik dari Ekstrak Anastatica hierochuntica L”, Biotropic : The Journal of Tropical Biology, 6(2), pp. 44–54. Available at: http://jurnalsaintek.uinsby.ac.id/index.php/biotropic/article/view/1573 (Accessed: 29 November 2022).