Identifikasi Bakteri Toleran terhadap Logam Berat Pb yang Diisolasi dari Air dan Sedimen di Sungai Porong, Sidoarjo

Authors

  • Nur Rokhmatul Lailiya Program Studi Biologi, Fakultas Sains dan Teknologi, UIN Sunan Ampel, Surabaya, Indonesia
  • Misbakhul Munir Program Studi Biologi, Fakultas Sains dan Teknologi, UIN Sunan Ampel, Surabaya, Indonesia
  • Esti Tyastirin Program Studi Biologi, Fakultas Sains dan Teknologi, UIN Sunan Ampel, Surabaya, Indonesia
  • Eko Teguh Pribadi Program Studi Biologi, Fakultas Sains dan Teknologi, UIN Sunan Ampel, Surabaya, Indonesia
  • Hanik Faizah Program Studi Biologi, Fakultas Sains dan Teknologi, UIN Sunan Ampel, Surabaya, Indonesia

Keywords:

Water and Sediment, Tolerant Bacteria, Pb heavy Metals, Lapindo Mud, Porong River

Abstract

Porong River is an active river located in the Sidoarjo region, East Java which has been polluted by the Lapindo mudflow containing various chemical compounds, one of which is lead (Pb) heavy metal. Isolation of bacteria in the environment contaminated with Pb allows bacteria that have the potential as bioremediation agents to grow. The purpose of this study was to determine the content of heavy metal Pb and isolate and identify lead (Pb) tolerant bacteria in water and sediment at Porong river, Sidoarjo. Sampling of water and sediment was carried out at 2 sampling points based on the presence of Lapindo mud waste flow. Analysis of the heavy metal content of Pb was carried out using the Atomic Absorption Spectrophotometer (AAS). Bacterial isolation was carried out on NA media containing 10 ppm Pb of heavy metal and incubated for 24 hours at 300C. Bacterial isolates were identified based on the macroscopic and microscopic properties and biochemical tests. The results of the heavy metal content of Pb at sampling point 1 in water was 0.81 ppm, and in sediment was 0.98 ppm while at sampling point 2 in water was 2.93 ppm, and in sediment was 3.88 ppm. The results of bacterial identification obtained as many as 6 bacterial isolates belonging to 3 genera of bacteria including Bacillus, Acinetobacter and Pseudomonas.

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References

Amriarni, A., Hendrarto, . B. & Hadiyarto, A., 2012. Bioakumulasi Logam Berat Timbal (Pb) Dan Seng (Zn) Pada Kerang Darah (Anadara granosa L.) dan Kerang Bakau (Polymesoda bengalensis L.) Di Perairan Teluk Kendari. Jurnal Ilmu Lingkungan, 9 (2), p. 45.

Anggraeni, D. S., 2017. Bioakumulasi Logam Berat Tembaga (Cu) Berdasarkan Waktu Paparannya Oleh Bakteri Endapan Sedimen Perairan Sekitar Rumah Susun Kota Makasar. Skripsi. UIN Alauddin Maksar.

Aryal, S., 2018. Urease Test-Principle, Media, Procedure and Result Interpretation. Diakses pada 15 Agustus 2020. https://microbioloyinfo.com.

Bangun, J., 2005. Kandungan Logam Berat Timbal (Pb) Dan Kadmium (Cd) Dalam Air, Sedimen dan Organ Tubuh Ikan Sokang (Triacanthus nieuhofi) di Perairan Ancol, Teluk Jakarta. Skripsi. Fakultas perikanan Dan Ilmu Kelautan. Intitut Pertanian Bogor.

Barrow, G. & A, F. R., 1993. Cowan and Steel's Manual for The Identification of Medical Bacteria. Cambridge: Cambridge University Press.

Breed, R., Murray, E. & R.S, N., 1957. Bergey's Manual of Determinative Bacteriology. 7th ed penyunt. USA: The Williams & Wilkins Company.

Brenner, D., Krieg, N. & J.T, S., 1925. Bergey's Manual of Systematic Bacteriology. 2nd penyunt. USA(Department of Microbiology and Molecular Genetics Michigan State University).

Cote, C., Heffron, J., Bozue, J. & Susan, L., 2015. Bacillus anthracis and Other Bacillus Species, Molecular Medical Microbiology: Second Edition. USAMRIC.

Cowan, 1993. Cowan and Steel's Manual for The Identification of Medical Bacteria. Third Edit penyunt. Cambridge: Cambridge University Press.

El-Sayed, M., 2016. Multiple Heavy Metal and Antibiotic Resistance of Acinetobacter baumannii Strain HAF – 13 Isolated from Industrial Effluents. American Journal of Microbiological Research, 4(1), pp. 26-36.

Fobes, S. & Weissfeld, 2007. Bailey & Scoot's Diagnostic Microbiology. St. Louis, Mo: Mosby, Inc.

Holt, e. a., 1994. Bergey's Manual of Determinative Bacteriologi. 9th penyunt. USA: Williams and Wilkins Baltimore.

Hafsan, S., 2014. Penuntun Praktikum Mikrobiologi Dasar. Universitas Alaudin Press.

Harlyan, L. i. & Sari, S. H. J., 2015. Konsentrasi Logam Berat Pb, Cu, dan Zn Pada Air dan Sedimen Permukaan Ekosistem Mangrove di Muara Sungai Porong, Sidoarjo, Jawa Timur. Perikananan Dan Kelautan, 20(1), pp. 53-60.

Husseuin, H., Ibrahim, S., Kendeel, K. & Moawa, H., 2004. Biosorption of Heavy Metals From Waste Water using Psedumonnas sp.. J. Biotechnol, 7(1).

Ika, I., Tahril, T. & Said, I., 2012. Analisis Logam Timbal (Pb) dan Besi (Fe) Dalam Air Laut Di Wilayah Pesisir Pelabuhan Ferry Taipa Kecamatan Palu Utara (The Analysis of Lead (Pb) and Iron (Fe) Metals in The Sea Water of Coastal Area of Taipaâs Ferry Harbor Subdistrict of North Palu). Jurnal Akademika Kimia, 1(4), pp. 181-186.

Kementerian Lingkungan Hidup Republik Indonesia, 2004. Peraturan Menteri Lingkungan Hidup Republik Indonesia Nomor 5.

Khoiroh, Z., 2014. Bioremediasi Logam Berat Timbal (Pb) Dalam Lumpur Lapindo Menggunakan Campuran Bakteri (Pseudomonas pseudomallei dan Pseudomonas aeruginosa). Skripsi. Universitas Islam Negeri Maulana Malik Ibrahim.

MacFaddin, J., 1980. Biochemical Test for Identification of Medical Bacteria. Second Ed penyunt. s.l.:Baltimore: Williams & Wilkins.

Panuntun, M. S., 2014. Isolasi dan Identifikasi Bakteri Toleran Timbal (Pb) dari Tanah Bekas Cetakan Pengecoran Logam di Desa Jeblokan, Kecamatan Ceper, Kabupaten Klaten. Skripsi. UIN Sunan Kalijaga.

Ratna, S., 2012. Mikrobiologi Dasar dalam Praktek. Jakarta: PT. Gramedia.

Rohma, N. S., 2017. Isolasi dan Identifikasi Bakteri Yang Berpotensi Sebagai Agen Bioremediasi Timbal (Pb) Dari Lumpur Lapindo. Skripsi. Universitas Islam Negeri Maualna Malik Ibrahim. Malang.

Suprapto, S. J., Gunardi, R. & Ramli, Y. R., 2007. Geokimia Sebaran Unsur Logam Pada Endapan Lumpur Sidoarjo. Buletin Sumber Daya Geologi, Volume 2, pp. 4-13.

Suwarto & Sarjani, E., 2014. Analisis Spasial Pencemaran Logam Berat Pada Sedimen dan Biota Air Di Muara Sungai. Geoedukasi Volume, III(1), pp. 30-37.

Tauran, P., Handayani, I. & N, S., 2013. Identifikasi Bakteri Aerob Gram Negatif dan Gram Positif Menggunakan Metode Konvensional dan Otomatik. Indones. J. Clin. Phatol. Med. Lab, 19(2), pp. 105-111.

Vogel, 1990. Analisis Anorganik Kualitatif Makro dan Semimikro. jakarta: PT. Kalman Media Pustaka.

Widiatmono, B. R., Dewi, L. S. & Agustianingrum, R., 2020. Bioremediasi Logam Berat Timbal (Pb) Menggunakan Bakteri Indigenous Pada Tanah Tercemar Air Lindih (Leachate). Jurnal Sumberdaya Alam dan Lingkungan.

Wulandari, S., Dewi, N. F. & Suwondo, 2005. Identifikasi Bakteri Pengikat Timbal (Pb) Pad Sedimen di Perairan Sungai Siak. Jurnal Biogenesis, 1(2), pp. 62-65.

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Published

2022-08-31

How to Cite

Lailiya , N. R. ., Munir, M. ., Tyastirin, E. ., Pribadi, E. T. . and Faizah, H. (2022) “Identifikasi Bakteri Toleran terhadap Logam Berat Pb yang Diisolasi dari Air dan Sedimen di Sungai Porong, Sidoarjo”, Biotropic : The Journal of Tropical Biology, 6(2), pp. 33–43. Available at: http://jurnalsaintek.uinsby.ac.id/index.php/biotropic/article/view/1571 (Accessed: 1 October 2022).

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