Pengaruh Pemberian Calcitriol terhadap Kadar Limfosit pada Tikus Putih (Rattus norvegicus) Galur Wistar Jantan yang Dipapar Asap Rokok
DOI:
https://doi.org/10.29080/biotropic.2021.5.1.29-40Keywords:
Cigarette smoke, Lymphocytes, calcitriolAbstract
The health impact for active and passive smokers is damage to the organs and respiratory tract. The body organs initially experience inflammation which increases the number of white blood cells, one of which is an increase in lymphocytes. One of the efforts to reduce the damage caused free radical by cigarette smoke is by consuming Calcitriol. Objective: To determine the effect of presenting Calcitriol on lymphocyte levels in male Wistar rats (Rattus norvegicus) exposed to cigarette smoke. Methods: This type of research is a pure experimental (true-experiment) using a pre and post-test research design with a control group design to check lymphocyte levels in male Wistar rats (Rattus norvegicus). 24 rats were divided into 6 groups simultaneously. Random, namely the control group 1 (exposed to cigarette smoke and not given Calcitriol), group 2 (exposed to cigarette smoke and not given Calcitriol), placebo control group (exposed to cigarette smoke and given olive oil), treatment 1 (exposed to cigarette smoke and given Calcitriol with a dose of 0.25 µgr / head), treatment 2 (exposed to cigarette smoke and given Calcitriol at a dose of 0.5 µgr / head) and treatment 3 (exposed to cigarette smoke and given Calcitriol at a dose of 1 µgr / head). Results: Paired t-test lymphocyte levels showed evidence that stated (p <0.05) in the K2 group (p = 0.014), the K3 group (p = 0.049), the P1 group (p = 0.012) and the P3 group (0.001) . Meanwhile, group K1 (p = 0.115) and group P2 (p = 0.066) had no statistical difference. Conclusion: Calcitriol administration can reduce lymphocyte levels in male Wistar rats exposed to cigarette smoke.
Downloads
References
Almatsier, S. 2010. Prinsip Dasar Ilmu Gizi. Gramedia Pustaka Utama, Jakarta.
Anto, R.j., Mukhopadhyay, A., Dening, K., Aggrawal, BB. 2002. Curcumin (diferuloyl – methane) Induces apoptosis through avtivation of caspase -8, BID cleavage and cyto chrome c realase. 23:143 – 150.
Asif, M., Karim, S., Umar, Z., Malik, A., Ismail, T., Chaudhary, A., Hussain, A. M., Rasool, M. 2013. Effect of cigarette smoking based on hematological parameters comparison between male smokers and non-smokers. Turkish Journal of Biochemistry/Turk Biyokimya Dergisi. Jan 1;38(1)
Aula, F.A., Qadir, F.A. 2012. Effect of cigarettesmoking on some immunological and hematological parameters in male smokers in Erbil City. Jordan Journal of Biological Science.6(2):159-66.
Bachmid, N. 2015. Uji aktivitas antikolesterol ekstrak etanol daun patikan emas (Euphorbia prunifolia Jacq.) pada Tikus Wistar yang hiperkolesterolemia. Jurnal MIPA. 4(1): 29-35.Balitbangkes RI. 2012. Global Adult Tobacco Survey: Indonesia report.
Brehm, J.M., Perez, A.E., Klei, L., Roeder, K., Barmada, M., Boutaoui, N., Forno, E., Kelly, R., Paul, K.m Sylvia, J., and Litonjua, A.A., 2012. Vitamin D insufficiency and severe asthma exacerbations in Puerto Rican Children. American Journal of respiratory and Critical care Medicine, 186 (2);140-146.
Darmalaksana, I.G.N., Sudimantini, L.M., Jayawarditha, A.A.G. dan Dada, I.K.A. 2018. Gerusan Daun Pegagan Mempercepat Kesembuhan Luka Bakar pada Tikus Putih. Buletin Veteriner Udayana. 2018;137-146.
Dewi, F.K. 2016 . Efek Ekstrak Kulit Buah Rambutan Terhadap Jumlah Eritrosit, Kadar hemoglobin, dan Hematokrit Tikus Putih yang Dipapar Asap Rokok.
Duso, A.D., Brown, A.J., Slatopolsky, E. 2005. Vitamin D. Am. J Physiol. Renal. Physiol. 289: 8-28.
Gil, A., Plaza, D.J., Mesa, M.D. 2018. Vitamin D Classic and novel actions. 72: 87-95.
Herlina, I., Purwanto, B., & Sugiarto, S. 2017. Pengaruh Pemberian 1, 25 Dihydroxyvitamin D (Calcitriol) Terhadap Kadar Fibroblast Growth Factor 23 Dan Albuminuria Pada Pasien Penyakit Ginjal Kronik Stadium V Yang Menjalani Hemodialisis. Biomedika, 9(1).
Hermawan, D. 2016. Sehat Selalu dengan Vitamin D. Penerbit Andi.
Huether, S.E., McCance, K.L. 2004. Understanding pathophysiology. 222-300.
Katzung, B.G. 2002. Basic and clinica Pharmacology.
Kementrian Kesehatan Republik Indonesia. 2010. Riset kesehatan dasar (Riskesdas). Badan Penelitian dan Pengembangan Kesehatan, Jakarta.
Ladas, E.J., Jacobson, J.S., Kennedy, D.D., Teel, K., Fleischauer, A., dkk. 2004. Antioxidants and cancer therapy a systematic review. Journal of clinical oncology. 22(3): 517-528.
Larasati, W. 2013. Uji Antifertilitas Ekstrak Etil Asetat Biji Jarak Pagar (Jatropha curas L.) pada tikus putih jantan (Rattus Norvegicus) Galur Sprague Dawley secara In Vivo. UIN Syarif Hidayatullah, Jakarta.
Leberl, M., Kratzer, A., & Taraseviciene, S.L. 2013. Tobacco smoke induced COPD/emphysema in the animal model—are we all on the Frontiers in physiology. 2013, (4)91
Mohri, M.K., Sharifi., Eidi, S. 2007. Hematology and serum biochemistri of Holstein dairy calves, Age related changes and comparison with blood composition in adults, Veterinary science. 83: 30 – 39.
Notoatmojo, S. 2012. Metodologi Penelitian Kesehatan. Rineka Cipta, Jakarta.
Rusmini, H., Fitriani, D., Hermawan, D., & Emilda, D. A. 2019. Pengaruh Vitamin D3 Terhadap Kadar Hemoglobin Tikus Wistar yang Dipapar Asap Rokok. Jurnal Ilmu Kesehatan, 1(1), 22-28.
Sen, S., Chakraborty, R., Sridhar, C., Reddy, Y.S.R., and De, B. 2010. Free radicals, antioxidants, diseases and phytomedicines, current status and future prospect. International journal of pharmaceutical sciences review and research. 3(1): 91-100.
Tanaka, T., & Takahashi, R. 2013. Flavonoids and asthma, Nutrients.5(6): 2128–2143.
World Health Organization. 2015. WHO global report on trends in prevalence of tobacco smoking.
Yulianto, E.A. 2015. Persepsi Siswa Smk Kristen (Ti) Salatiga Tentang Bahaya Merokok Bagi Kesehatan. J Phys Educ.4(5);1807–13.
